Lipid homeostasis depends on membrane contact sites (MCS). This study shows that the integrity of multiple ER MCS requires Pex30 binding to phosphatidic acid via its dysferlin domain. The authors also show that Pex30 activity is regulated by phosphorylation in response to metabolic cues.

In this study, we show that Pex30 dysferlin domain binds PA through its two hydrophobic regions adjacent to beta sheets. We propose that, PA, a precursor for neutral lipids, recruits Pex30 at ER subdomains that plays a critical role in LD biogenesis.

The γ-TuRC is recruited to microtubule-organizing centers by NEDD1. Muñoz-Hernández et al. report cryo-EM structures revealing how NEDD1’s C terminus forms an α-helical tetramer that docks onto the γ-TuRC without inducing major conformational changes in the complex, compatible with CDK5RAP2 binding and γ-tubulin ring closure.

Centriolar satellites are cytoplasmic particles found concentrated near centrosomes and the base of cilia in vertebrate cells whose functional contribution has long been unclear. Here, Pachinger et al. present a model for satellites not as transport modules but as sites of translation specifically of centrosomal and ciliary proteins.

This study provides insights into how cargo adaptors bind myosin V. Genetics, cell-based assays, cryo-EM, and AlphaFold reveal that the vacuole-specific adaptor uses a handhold mechanism to attach to two areas on the myosin-V tail. Moreover, evidence is presented that other adaptors use a similar strategy.

Human cells repair plasma membrane lesions in a two-step process. After a membrane scab forms at the lesion site, the scab is shed as annexin-containing extracellular vesicles.

The precise roles of the mitophagy receptors BNIP3 and NIX remain unclear. This study demonstrates that they are required for tight attachment and expansion of the isolation membrane along the mitochondrial surface, supporting a characteristic morphologic feature of the mitophagy.