Cryo-EM processing pipeline. (A) Summary of the new rec-γ-TuRC cryo-EM data processing strategy. Cryo-EM collection details were reported in Aher et al. (2024). (B) Gold-standard Fourier shell correlation (FSC) plot for the consensus rec-γ-TuRC density map. The FSC at 0.143 is indicated by a dashed line. (C) Summary of new rec-γ-TuRC + sDK5RAP2 cryo-EM data processing strategy. Cryo-EM collection details for datasets 1–4 (marked by asterisks) were reported in Xu et al. (2024). (D) Gold-standard FSC plot for the consensus rec-γ-TuRC + CDK5RAP2 density map. The FSC at 0.143 is indicated by a dashed line. (E) Two views of the consensus rec-γ-TuRC density map analyzed by CryoSPARC, showing a resolution distribution ranging from 2.5 to >12.5 Å. (F) Two views of the particle angular distribution overlaid onto the rec-γ-TuRC consensus map. (G) Two views of the consensus rec-γ-TuRC + CDK5RAP2 density map analyzed by CryoSPARC, showing a resolution distribution ranging from 2.5 to >12.5 Å. (H) Two views of the particle angular distribution overlaid onto the rec-γ-TuRC + CDK5RAP2 consensus map.