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Figure S4.
MCS regulated by Pex30 complexes contribute to normal PA distribution. (A) Percentage of the membrane lipid type in whole-cell lysates from cells in the stationary growth phase with the indicated genotype. The bars represent the mean and SD. One-way ANOVA and Dunnett’s multiple comparisons were performed to compare the percentage of each lipid type with the WT condition (****P < 0.0001; **P < 0.01; ns, not significant). (CDP-DAG: Cytidine Diphosphate Diacylglycerol; CL: Cardiolipin; Cer: Ceramide; DAG: Diacylglycerol; EE: Sterol Ester; Erg: Ergosterol; IPC: Inositol Phosphorylceramide; LPA: Lyso-Phosphatidic Acid; LPC: Lysophosphatidylcholine; LPE: Lysophosphatidylethanolamine; LPI: Lysophosphatidylinositol; LPS: Lysophosphatidylserine; M(IP)2C: Mannosyl di-(inositolphosphoryl)-ceramide; MIPC: Mannosyl inositolphosphoryl-ceramide; PA: Phosphatidic Acid; PC: Phosphatidylcholine; PE: Phosphatidylethanolamine; PG: Phosphatidylglycerol; PI: Phosphatidylinositol; PS: Phosphatidylserine; TAG: Triacylglycerol). (B) Same as A but in membranes from purified vacuoles from cells with the indicated genotype. The bars represent the mean and SD. One-way ANOVA and Dunnett’s multiple comparisons were performed to compare the percentage of each lipid type with the WT condition (****P < 0.0001; **P < 0.01; *P < 0.05; ns, not significant). (C) Localization of Spo2051–91-GFP-ER in cells with mutations in Pex30 family members. Cells were analyzed during the diauxic shift after overnight growth in SC medium. Individual Z-planes corresponding to the center and the periphery of the cell are shown. Bar, 5 µm. (D) Localization of Spo2051–91-GFP-ER in cells with mutations on the tether proteins of ER–peroxisome MCS and NVJ. Cells were analyzed during the diauxic shift after overnight growth in SC medium. Individual Z-planes corresponding to the center and the periphery of the cell are shown. Bar, 5 µm. (E) Localization of Spo2051–91-GFP-ER in cells with mutations not related to Pex30-related MCS. Cells were analyzed during the diauxic shift after overnight growth in SC medium. Individual Z-planes corresponding to the center and the periphery of the cell are shown. Bar, 5 µm.

MCS regulated by Pex30 complexes contribute to normal PA distribution. (A) Percentage of the membrane lipid type in whole-cell lysates from cells in the stationary growth phase with the indicated genotype. The bars represent the mean and SD. One-way ANOVA and Dunnett’s multiple comparisons were performed to compare the percentage of each lipid type with the WT condition (****P < 0.0001; **P < 0.01; ns, not significant). (CDP-DAG: Cytidine Diphosphate Diacylglycerol; CL: Cardiolipin; Cer: Ceramide; DAG: Diacylglycerol; EE: Sterol Ester; Erg: Ergosterol; IPC: Inositol Phosphorylceramide; LPA: Lyso-Phosphatidic Acid; LPC: Lysophosphatidylcholine; LPE: Lysophosphatidylethanolamine; LPI: Lysophosphatidylinositol; LPS: Lysophosphatidylserine; M(IP)2C: Mannosyl di-(inositolphosphoryl)-ceramide; MIPC: Mannosyl inositolphosphoryl-ceramide; PA: Phosphatidic Acid; PC: Phosphatidylcholine; PE: Phosphatidylethanolamine; PG: Phosphatidylglycerol; PI: Phosphatidylinositol; PS: Phosphatidylserine; TAG: Triacylglycerol). (B) Same as A but in membranes from purified vacuoles from cells with the indicated genotype. The bars represent the mean and SD. One-way ANOVA and Dunnett’s multiple comparisons were performed to compare the percentage of each lipid type with the WT condition (****P < 0.0001; **P < 0.01; *P < 0.05; ns, not significant). (C) Localization of Spo2051–91-GFP-ER in cells with mutations in Pex30 family members. Cells were analyzed during the diauxic shift after overnight growth in SC medium. Individual Z-planes corresponding to the center and the periphery of the cell are shown. Bar, 5 µm. (D) Localization of Spo2051–91-GFP-ER in cells with mutations on the tether proteins of ER–peroxisome MCS and NVJ. Cells were analyzed during the diauxic shift after overnight growth in SC medium. Individual Z-planes corresponding to the center and the periphery of the cell are shown. Bar, 5 µm. (E) Localization of Spo2051–91-GFP-ER in cells with mutations not related to Pex30-related MCS. Cells were analyzed during the diauxic shift after overnight growth in SC medium. Individual Z-planes corresponding to the center and the periphery of the cell are shown. Bar, 5 µm.

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