DysF domains of Pex30 adaptors are also required for complex localization. (A) Crude membrane fractions of cells expressing endogenously tagged variants of Pex28-13xMyc, Pex29-V5, and Pex32-3xHA, or untagged proteins as control, were solubilized with detergent, and the extracts were subjected to immunoprecipitation (IP) with anti-Myc, V5, or HA antibodies. Eluted proteins were separated by SDS-PAGE and analyzed by western blotting. Pex28-Myc, Pex29-V5, Pex30, and Pex32-HA were detected with anti-Myc, anti-V5, anti-Pex30, and anti-HA, respectively. Dpm1, used as a loading control, was detected with anti-Dpm1 antibody. *, IgG light chain. IB, immunoblot. The position of molecular weight markers (in kDa) is indicated. (B) Localization of endogenous Pex29-mNG variants and Nvj1-tdTomato in cells during the diauxic shift. Yellow arrowheads highlight colocalization between Pex29 and Nvj1, and magenta arrowheads highlight the non-concentrated Nvj1. Bar, 5 µm. (C) Localization of endogenous Pex32-mNG variants and Inp1-mCherry in exponentially growing cells. Bar, 5 µm. Source data are available for this figure: SourceData FS2.