Satellite scaffolding component PCM1 is conserved beyond vertebrates. (A) Schematic representation of the transport model of centriolar satellite (magenta) function as mediators of dynein-dependent recruitment of centrosomal and ciliary client proteins (blue) for centrosome/cilium biogenesis. (B) Reciprocal BLAST analysis reveals the presence of PCM1 orthologs across opisthokonts, correlating with the reported presence of centriole-organized centrosomes and cilia (Azimzadeh, 2014; Grell and Benwitz, 1981). (C) Multiple sequence alignment of conserved C terminus (part of pfam15717) of selected PCM1 orthologs. Note that Drosophila Combover (CMB) is highly divergent. (D) Overexpression but not RNAi-mediated depletion or mutation of Cmb results in a PCP phenotype, seen by misalignment of bristles in the fly notum. Tissue-specific RNAi was performed using the Pannier-GAL4 driver, with the PCP effector Fritz (Strutt and Warrington, 2008) as a positive control. See also Fig. S1.