Peroxisome inheritance requires that the Inp2 adaptor binds to two distinct sites on Myo2. (A) Residues on the Myo2 tail identified as important for peroxisome inheritance in vivo (solid outline) are distinct from those observed in structural studies (dashed outline) of the Inp2 peptide(532–540) in complex with the Myo2 tail (Tang et al., 2019; Fagarasanu et al., 2006, 2009). (B) Myo2 residues implicated in Inp2 interaction—through genetic studies, Myo2(W1407), or structural analysis, Myo2(F1264) and Myo2(F1275)—were tested for their role in peroxisome inheritance. Data were analyzed using ordinary one-way ANOVA with a multiple comparisons test (n = 3 independent experiments, with each n including ≥40 cells per bud size per group). ****P < 0.0001; **P < 0.01; ns = not significant. YFP-PTS1 encodes YFP fused with a peroxisome-targeting sequence. Scale bar = 5 μm.