Data associated with Fig. 6 . (A) Phospholipid measurements of indicated strains taken by LC-HRMS (n = 3). Distribution of total quantitated phospholipids by class (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001). (B) WF images of the indicated strains endogenously expressing Pex30-2xmCherry. Cells were grown in synthetic media or synthetic media with 1 mM ethanolamine supplementation. Yellow arrowheads denote Pex30 accumulation. Bar = 4 μm. (C) Quantification of experiment from B showing percent cells with Pex30 accumulation. Bars show mean from two independent experiments and SEM. 100 cells per genotype from each replicate were analyzed. (D) WF images of the indicated strains endogenously expressing Pex30-2xmCherry and Opi1-GFP on a plasmid in logarithmic phase. Bar = 4 μm. (E) Quantification of experiment from D showing percent cells with Pex30-2xmCherry accumulation and Opi1-GFP accumulation in each genotype. Bars show mean from three independent experiments and SEM. 100 cells per genotype from each replicate were analyzed and compared using one-way ANOVA and Tukey’s multiple comparison test. (F) Phospholipid measurements of indicated strains taken by LC-HRMS (n = 3). Amount of total quantitated PA relative to total quantitated phospholipids (**P < 0.01, ***P < 0.001, and ****P < 0.0001).. WF, widefield images; LC-HRMS, liquid-chromatography high-resolution mass spectrometry.