Figure S2. Data associated with Fig. 4 ,... | Rockefeller University Press

Figure S2.

$Data associated withFig. 4. (A) SDS-PAGE gel stain with Coomassie blue simple stain. Protein ladder (M), lysate (S), flow through (FT), 1–20 wash, 1–4 elution with imidazole, and size exclusion chromatography (SEC). (B) Size exclusion chromatography. Peak 4 was collected and used for liposome flotation assays. (C) Protein fraction from B was immunoblotted using anti-6xHis antibody to check for the presence of 6xHis-DysF domain. (D) Protein-lipid overlay assay. (E) WF images of WT and sei1Δ cells endogenously expressing Pex30-2xmCherry and FYVE-GFP on a plasmid in logarithmic phase. Cells were stained with MDH for LDs. White arrowheads denote Pex30, LD, and FVYE puncta colocalization. Bar = 4 μm. (F) As in Fig. 4 C, but with different PA species. Source data are available for this figure: SourceData FS2.. WF, widefield images.$

Data associated with Fig. 4,. (A) SDS-PAGE gel stain with Coomassie blue simple stain. Protein ladder (M), lysate (S), flow through (FT), 1–20 wash, 1–4 elution with imidazole, and size exclusion chromatography (SEC). (B) Size exclusion chromatography. Peak 4 was collected and used for liposome flotation assays. (C) Protein fraction from B was immunoblotted using anti-6xHis antibody to check for the presence of 6xHis-DysF domain. (D) Protein-lipid overlay assay. (E) WF images of WT and sei1Δ cells endogenously expressing Pex30-2xmCherry and FYVE-GFP on a plasmid in logarithmic phase. Cells were stained with MDH for LDs. White arrowheads denote Pex30, LD, and FVYE puncta colocalization. Bar = 4 μm. (F) As in Fig. 4 C, but with different PA species. Source data are available for this figure: SourceData FS2.. WF, widefield images.

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