DysF domain binds PA. (A) Diagram of the liposome flotation assay based on density (Nycodenz) gradient centrifugation. (B and C) 12 fractions of 62.5 μl each were collected and subjected to western blot using anti-6xHis monoclonal antibody to check for the presence of 6xHis-DysF. Source data are available for this figure: SourceData F4. PE, phosphatidylethanolamine.