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Figure 2.
PA accumulation at LD biogenesis sites precedes Pex30 accumulation. (A) AS images of are1are2dga1sei1Δ-GAL1-LRO1 cells endogenously expressing Pex30-2xmCherry and Opi1-GFP on a plasmid. Cells were stained for LDs with MDH and imaged at indicated time intervals after LD induction. Bar = 4 μm. (B) Quantification of experiment in A showing the percent of cells with Pex30-2xmCherry accumulation, Opi1-GFP accumulation, and LDs over time. Time points represent the mean of three independent experiments. 100 cells per time point from each replicate were analyzed. Error bars represent SEM. (C) Quantification of experiment in A showing LD association with Pex30-2xmCherry puncta, Opi1-GFP accumulation, and Pex30-2xmCherry accumulation over time from three independent experiments. 50 LDs per time point from each replicate were analyzed and bars show the mean and SEM. AS, Airyscan images.

PA accumulation at LD biogenesis sites precedes Pex30 accumulation. (A) AS images of are1are2dga1sei1Δ-GAL1-LRO1 cells endogenously expressing Pex30-2xmCherry and Opi1-GFP on a plasmid. Cells were stained for LDs with MDH and imaged at indicated time intervals after LD induction. Bar = 4 μm. (B) Quantification of experiment in A showing the percent of cells with Pex30-2xmCherry accumulation, Opi1-GFP accumulation, and LDs over time. Time points represent the mean of three independent experiments. 100 cells per time point from each replicate were analyzed. Error bars represent SEM. (C) Quantification of experiment in A showing LD association with Pex30-2xmCherry puncta, Opi1-GFP accumulation, and Pex30-2xmCherry accumulation over time from three independent experiments. 50 LDs per time point from each replicate were analyzed and bars show the mean and SEM. AS, Airyscan images.

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